Type: TLC Application #: 400160
Detection: ninhydrin/cadmium acetate spray reagent (see chapter visualization reagents) Conditions: Eluent: citrate buffer pH 3.3 (84 g citric acid monohydrate, 16 g NaOH, 5.9 ml HCl 37% per litre) Migration distance: 15 cm in 2 h Authors: Devenyi T Source: Hungarian Scientific Instruments 30 (1974) 13 and Acta Biochim et Biophys Acad Sci Hung 11 (1976) 1
Details
Legend: Detection of the amino acid spectrum in peptide hydrolysates by 1-dimensional
TLC on cation exchanger
layers in citrate buffer pH 3.3. K = reference solution, 1, 2 and 3 = peptide
hydrolysates